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How Do You Automate a Multicolumn Protein Purification?

Multicolumn purifications are tedious and the manual operations during this process introduce variabilities, affecting the success of purification and reproducibility of results. This whiteboard animation explains how an automated multi-dimensional chromatography method can make this an easier process with very high success rates.
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ArticlesChromatographyTechnical Reports

Mixed-Mode Chromatography for mAb S Aggregate Removal: Comparison of CHT™ Ceramic Hydroxyapatite, Capto adhere, and Capto adhere ImpRes

The separation of monomers from product-related impurities is quite challenging in process separations. It requires a thorough screening and an efficient resin to get efficiency in the purification process. Review the performance data of Bio-Rad’s mixed mode CHT™ Ceramic Hydroxapatite in achieving process separation efficiency and how it compares with various Capto media.
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How Does the Mixed Mode Interaction of a Media Help Achieve High Selectivity and Resolution?

The best chromatography media should yield high protein purity and product recovery as well as be versatile enough to purify difficult samples. Bio-Rad’s CHT™ Ceramic Hydroxyapatite achieves this by virtue of being able to interact with the proteins through multiple modes, making the process extremely efficient. The whiteboard animation describes the mode of action of CHT.
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ArticlesChromatographyProtocols and Tips

Chromatography: Everything You Need to Know about Your Peaks

What can chromatography peaks tell us? From the quality of column packing to the purity of separated proteins, a lot can be gleaned from the peaks. Here are some definitions that can help decipher what chromatography peaks can tell us.
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ArticlesChromatographyProduct Highlights

Bio-Rad’s Multi-D Method Addresses Unmet Chromatography Needs

Some of the frustrations in protein purification stem from unmet chromatography needs, such as automation, productivity, throughput, and reproducibility among others. Bio-Rad’s new Multi-D chromatography method addresses these and provides solutions to faster, efficient and more reliable protein purification.
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ArticlesChromatographyTechnical Reports

Advantages of Multidimensional (Multi-D) Chromatography Using the NGC™ Chromatography System over Traditional Sequential Chromatography

Traditional sequential chromatography typically requires intensive hands-on user manipulation, which could hinder the reproducibility of protein purification runs. Multi-D chromatography aims to improve this process while maintaining the efficacy of the traditional workflow.
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AppsChromatography

NGC™ System Tour and Configurator

The NGC Chromatography System introduces a new level of modularity and usability for protein purification. The modular design of the NGC System provides multiple configurations, each addressing different requirements. Use our configurator tool to build your own system to meet your specific workflow and throughput needs.
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ArticlesChromatographyResearch Highlights

Purification Trends: How Smart is Chromatography Automation?

What does automated protein purification mean today? What might it look like tomorrow? Bioradiations talks to a variety of users about what they’re doing now to implement automation in their purification processes, and what possibilities they envision that will improve chromatography in the near future.
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ArticlesChromatographyTechnical Reports

Protein Purification Workflow Development Using Bio-Rad’s NGC™ Chromatography System

While using affinity tagging is the preferred method to achieve highly pure protein through recombinant expression, this approach isn’t feasible for all proteins. This tech report describes workflow optimizations using the NGC Chromatography System that allow users to achieve high purities with untagged proteins.
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ArticlesChromatographyCustomer Stories

Unlocking the Structures of Natural Killer Cell Receptors with the NGC™ Chromatography System

Structural biologist Petr Novák employs the latest techniques to model natural killer cell receptor molecules, research that may one day result in powerful therapies to treat cancer and infections. Go inside the fascinating world of structural characterization and find out how Novák has integrated the NGC™ chromatography system into his effort to visualize these molecules in vivid detail.
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