See how one study used Bio-Rad’s Bio-Plex Pro Human Cytokine 27-Plex Assay to investigate immune responses to an anti-viral compound in an effort to better understand vaccine efficacy.
Follow the five tips in this article before embarking on your multiplex immunoassay journey to generate accurate, efficient, and biologically relevant results. From help designing experiments to acquiring accurate data, this article has it all.
Multiplex immunoassay development requires a lot of validation and standardization steps. Several factors can influence the performance of these assays. Some factors that need to be considered are mentioned here.
How can we easily identify biomarkers for different immune cell types? This color-coded poster makes identification of mouse lineage biomarkers easy.
Bio-Rad’s Bio-Plex Pro Human Inflammation Assay measures 37 of the most relevant inflammation biomarkers, including TNF superfamily proteins, Treg cytokines, IFN proteins, and matrix metalloproteinases, in a single multiplex panel.Check out the performance characteristics of the assay panel in this report.
Mice are an optimal model organism for human cancer studies. However, when experiments involve a human xenograft in a mouse host, cross-reactivity presents a major concern in measuring biomarkers. This technical report evaluates 14 Bio-Plex Pro Human Cancer Biomarker Assays for cross reactivity to mouse proteins and discusses the resulting data.
Bio-Rad’s new Bio-Plex Pro RBM Apoptosis Multiplex Assays enable complex, quantitative analyses of biomarkers in the intrinsic apoptosis pathway. Learn more about these biomarkers and view sample data and performance characteristics of these assays in this detailed technical report.
Applications of Bio-Plex Pro™ Cell Signaling Akt and MAPK Assay Panels in Signal Transduction Pathways
The Akt and MAPK signal transduction pathways are key areas of study for their roles in cell growth, function, and death, as well as for their possible roles in cancer development and diabetes. Bio-Rad’s new Bio-Plex Pro™ Akt and MAPK cell signaling assays enable researchers to study the activation of these key pathways as a response to various stimuli. This technical report validates the sensitivity and specificity of these assays after cells were exposed to insulin, EGF (epidermal growth factor), and UV radiation.
Profiling Dynamic Cancer Drug Responses with BioMarker Strategies, LLC and the Bio-Plex® Multiplex Immunoassay System
In an age of personalized medicine, getting the most out of highly targeted cancer therapies takes finding the best molecular targets for each patient. BioMarker Strategies LLC, a small startup, has been developing an innovative method to do this by modeling oncogenic pathway responses, but they needed to accurately measure the levels of phosphoproteins resulting from the activation of these genes. Learn how they are using the Bio-Plex multiplex immunoassay system to accurately quantify the proteins and generate functional signaling profiles for these oncogenes.