The iTaq™ universal SYBR® Green supermix and iTaq™ universal probes supermix are ready-to-use 2x supermixes formulated for sensitive, efficient, and reproducible qPCR data on any instrument.

iTaq universal supermixes let you:

  • Obtain reliable and reproducible gene expression analysis results
  • Perform challenging simplex or duplex assays for a wide variety of targets (probes supermix)
  • Achieve consistent results across real-time instrument platforms under fast or standard conditions

iTaq universal supermixes contain:

  • Advanced buffer formulation for optimal dye-based or probe-based qPCR
  • Antibody-mediated hot-start iTaq DNA polymerase for rapid 30 sec activation
  • A blend of passive reference dyes for universal instrument compatibility

Performance data for the iTaq™ universal SYBR® Green supermix and the iTaq universal probes supermix are presented below.

iTaq™ Universal SYBR® Green Supermix Performance Data

High Reproducibility and Robust Discrimination

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Fig. 1. High reproducibility and robust discrimination. Exceptional reproducibility can be achieved with iTaq™ universal SYBR®Green supermix. Efficient discrimination and reliable quantification can be obtained from a 1.33-fold serial dilution of input template. The β-actin gene was amplified from varying amounts of HeLa cDNA (1 ng to 136 pg). From left to right: 1 ng, 753 pg, 565 pg, 425 pg, 320 pg, 240 pg, 181 pg, and 136 pg. Inset is a magnified view showing robust discrimination and reproducible amplification (six replicates for each input amount). RFU, relative fluorescence units.

Reliable Gene Expression Analysis

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Fig. 2. Reliable gene expression analysis. iTaq™ universal SYBR® Green supermix provides reliable gene expression data. The β-actin gene was amplified from HeLa cDNA (100 ng to 100 fg) using the CFX96™ real-time PCR detection system. iTaq™ universal SYBR® Green supermix showed over 90% efficiency over six orders of linear dynamic range (R2 = 0.999, slope = –3.512, actin efficiency = 92.6%) RFU, relative fluorescence units.

Sensitive and Efficient Amplification of Genomic DNA

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Fig. 3. Extreme sensitivity. iTaq™ universal SYBR® Green supermix allows robust amplification of genomic DNA. The human GAPDH gene was amplified from gDNA (50 ng to 5 g) using the CFX96 real-time PCR detection system. iTaq™ universal SYBR® Green supermix showed 108.1% GAPDH efficiency over several orders of linear dynamic range (R2 = 0.993, slope = –3.143). RFU, relative fluorescence units.

iTaq Universal Probes Supermix Performance Data

Efficient Gene Expression Analysis

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Fig. 1. Efficient gene expression analysis. iTaq universal probes supermix is excellent for gene expression analysis. The human β-actin gene was amplified using FAM-labeled probes from HeLa cDNA (100 ng to 100 fg) using the CFX96 real-time PCR detection system. iTaq universal probes supermix showed 94.3% actin efficiency over 6 orders of linear dynamic range (R2 = 0.999, slope = –3.467). RFU, relative fluorescence units.

Reproducible Quantification

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Fig. 2. Reproducible quantification. Exceptional reproducibility can be achieved with iTaq universal probes supermix. Efficient discrimination and reliable quantification can be obtained from a 1.33-fold serial dilution of input template. The β-actin gene was amplified from varying amounts of HeLa cDNA (1 ng to 136 pg). From left to right: 1 ng, 753 pg, 565 pg, 425 pg, 320 pg, 240 pg, 181 pg, and 136 pg. Inset is a magnified view showing robust discrimination and reproducible amplification (six replicates for each input amount). RFU, relative fluorescence units.

Extreme Sensitivity

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Fig. 3. Extreme sensitivity — efficient amplification of low-abundance target. iTaq universal probes supermix allows efficient amplification and accurate detection of low-abundance targets. The IL-1β gene was amplified using FAM-labeled probes from HeLa cDNA (100, 10, 1, and 0.1 ng) using the CFX96 real-time PCR detection system. iTaq univesal probes supermix showed sensitive detection IL-1β gene even with very low cDNA inputs. RFU, relative fluorescence units.

Duplex qPCR Under Challenging Conditions

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Fig. 4. Duplex qPCR under challenging conditions. Gene expression analysis of two targets using iTaq universal probes supermix on the CFX96 real-time PCR detection system. cDNA from human liver (100 ng) was used in each 20 µl reaction for the amplification of tubulin (HEX-labeled probe ) and IL-2 (FAM–labeled probe ) both in simplex (A) and duplex (B) reactions. There are no significant differences in quantification cycles (Cqs) in the detection of a low-expressing gene (IL-2) in a duplex or singleplex reaction in the presence of a high-expressing gene (GAPDH). RFU, relative fluorescence units.

Ordering Information

Catalog# Description
172-5120 iTaq™ Universal SYBR® Green Supermix, 2 ml (2 x 1 ml vials), 200 x 20 µl reactions
172-5121 iTaq™ Universal SYBR® Green Supermix, 5 ml (5 x 1 ml vials), 500 x 20 µl reactions
172-5122 iTaq™ Universal SYBR® Green Supermix, 10 ml (10 x 1 ml vials), 1000 x 20 µl reactions
172-5124 iTaq™ Universal SYBR® Green Supermix, 25 ml (5 x 5 ml vials), 2500 x 20 µl reactions
172-5125 iTaq™ Universal SYBR® Green Supermix, 50 ml (10 x 5 ml vials), 5000 x 20 µl reactions
172-5130 iTaq Universal Probes Supermix, 2 ml (2 x 1 ml vials), 200 x 20 µl reactions
172-5131 iTaq Universal Probes Supermix, 5 ml (5 x 1 ml vials), 500 x 20 µl reactions
172-5132 iTaq Universal Probes Supermix, 10 ml (10 x 1 ml vials), 1000 x 20 µl reactions
172-5134 iTaq Universal Probes Supermix, 25 ml (5 x 5 ml vials), 2500 x 20 µl reactions
172-5135 iTaq Universal Probes Supermix, 10 ml (10 x 5 ml vials), 5000 x 20 µl reactions

SYBR is a trademark of Molecular Probes, Inc. Bio-Rad Laboratories, Inc. is licensed by Molecular Probes, Inc. to sell reagents containing SYBR Green I for use in real-time PCR, for research purposes only.

Bio-Rad’s real-time thermal cyclers are licensed real-time thermal cyclers under Applera’s U.S. Patent Number 6,814,934 B1 for use in research, human in vitro diagnostics, and all other fields except veterinary diagnostics.

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