Small vesicles were identified over 50 years ago but scientists are still trying to understand their biology and role in disease. These vesicles have become relevant for monitoring, diagnosing, and treating complex diseases such as cancer. Yet, because of their small size compared to cells, they require intricate technologies to truly understand their impact in research. Learn about the various technologies used to study small vesicles and discover the power of flow cytometry for learning more about these valuable vesicles.
Extracting proteins from tissue lysates for western blotting can be a challenge. From knowing how to process your tissue to verifying protein loading to including suitable controls, there is a lot to keep track of. The ten tips in this article can help you on your way to generating reproducible and reliable blots and do better western blotting overall.
Normalization of western blot data is very crucial in quantitating proteins. Normalization of band intensity of proteins of interest with the band intensity of housekeeping proteins (HKP) is routinely done in labs. But more and more journals question the validity of using HKPs in normalization and are demanding more validations and tests. An alternative method is to use total protein normalization (TPN) for normalization. Explore the TPN option and see how you can get reliable western blot data easily.